Utilizing Chick Embryo in Ovo Model System to Study the Effects of Misoprostol on Early Embryogenesis - A Pilot Study

The current study reports preliminary results of a pilot project designed for evaluating the effects of misoprostol administration on developing chick embryos. The project comprised of two groups of fertilized chicken eggs. The groups were labelled as “A” for control (n=5) and “B” for treated (n = 5) specimens. The treated group embryos were exposed to 4 μg of misoprostol by injecting the drug directly into the yolk sac, while same volume of normal saline was injected in the control group. Whole mount examination of sacrificed embryos after 33 hours of incubation revealed normal stage of development (HHstage 10) in the control group embryos. While in the treated group, 02 out of 05 embryos lagged in their developmental stage (HHstage 8), with no brain vesicles formation and lesser number of somite pairs.